Optimization of the genetic code expansion technology for intracellular labelling and single-molecule tracking of proteins in genomically re-coded <i>E. coli</i>

2025/11/24 by Filip Ilievski, Linnea Wikström, Anneli Borg +3 · 1 voice
Biochemistry, Genetics and Molecular Biology · Chemistry · Medicine · #Advanced Fluorescence Microscopy Techniques #Click Chemistry and Applications #Monoclonal and Polyclonal Antibodies Research

paper · pdf · doi:10.1039/d5cb00221d

openalex publication_date 2025/11/24 · openalex created_date 2025/11/25 · openalex updated_date 2026/07/02

Abstract

Genetic code expansion (GCE) technology has been optimized, applied, and validated for single-molecule tracking (SMT) of intracellularly dye-labelled proteins in a fully re-coded E. coli strain.

Citations

• A Strain-Promoted [3 + 2] Azide−Alkyne Cycloaddition for Covalent Modification of Biomolecules in Living Systems
• Click Chemistry: Reaction Rates and Their Suitability for Biomedical Applications
• Dynamic binding of the bacterial chaperone Trigger factor to translating ribosomes in <i>Escherichia coli</i>

Discussions

• ✨New advance article! Johansson et al. introduce FLORENCE, Fluorescence labelling in recoded E.coli with non-canonical chemical entities, a SMT labelling method based on genetic code expansion offeri [bsky, 1 points, 0 comments]

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