2024/11/15 by Peter T. Rühr, Johannes Strauß · 1 voice
paper · doi:10.5281/zenodo.14168668
openalex publication_date 2024/11/15 · openalex created_date 2026/01/11 · openalex updated_date 2026/07/22
Full resolution µCT scan of the midleg tibia of Bacillus rossius (Rossi, 1790) analysed in the following publication: Strauß, J. & Rühr, PT (2026): The Mechanosensory Subgenual Organ Complex in the Stick Insect Bacillus rossius (Phasmatodea): Neuroanatomy and Functional Morphology. The Journal of Comparative Neurology 534(1). doi: 10.1002/cne.70126 Fixation Bouin's solution in 70% EtOH Staining 12 days in 0.3% phosphotungstic acid (PTA) solution in 70% EtOH Washing, storage and scanning 70% EtOH Device Skyscan 1272 (Bruker microCT, Kontich, Belgium) at BIOB, University of Bonn Tube voltage 40 kV Tube current 200 µA Sample rotation 360° Step size 1° Exposure time 1000 ms Binning 2x2 Filter none Averaging 4 Random movement 3 Projections 3600 + reference scans Voxel size 1 µm Thermal drift correction and digital section reconstruction NRecon v.2.2 (Bruker microCT, Kontich, Belgium) Cropping and CLAHE¹ filtering Fiji² with macro³ ¹ Zuiderveld, K. (1994). Contrast limited adaptive histogram equalization. In Graphics gems IV (pp. 474–485). USA: Academic Press Professional. doi: 10.1016/B978-0-12-336156-1.50061-6 ² Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., et al. (2012): Fiji: an open-source platform for biological-image analysis. Nature Methods, 9(7), 676–682. doi: 10.1038/nmeth.2019 ³ Fischer, S. (2019, June 12). Apply CLAHE filter on a stack in Fiji – Electron microscopy / Image analysis / 3D reconstruction. http://stefischer.de/2019/06/12/apply-clahe-filter-on-a-stack-in-fiji/. Accessed 15 November 2024