2026/01/22 by Daria Meyer, Winfried Göettsch, Jannes Spangenberg +10 · 1 voice · 1 citation
Biochemistry, Genetics and Molecular Biology · Engineering · #Genomics and Phylogenetic Studies #Nanopore and Nanochannel Transport Studies #Single-cell and spatial transcriptomics
paper · doi:10.1093/nar/gkag023
openalex publication_date 2026/01/22 · openalex created_date 2026/02/03 · openalex updated_date 2026/07/31
Nucleic acid sequencing is the process of identifying the sequence of DNA or RNA, with DNA used for genomes and RNA for transcriptomes. Deciphering this information has the potential to greatly advance our understanding of genomic features and cellular functions. In comparison to other available sequencing methods, nanopore sequencing stands out due to its unique advantages of processing long nucleic acid strands in real time, within a small portable device, enabling the rapid analysis of samples in diverse settings. Evolving over the past decade, nanopore sequencing remains in a state of ongoing development and refinement, resulting in persistent challenges in protocols and technology. This article employs an interdisciplinary approach, evaluating experimental and computational methods to address critical gaps in our understanding in order to maximize the information gain from this advancing technology. Here, we present both overview and analysis of all aspects of nanopore sequencing by providing statistically supported insights. Thus, we aim to provide fresh perspectives on nanopore sequencing and give comprehensive guidelines for the diverse challenges that frequently impede optimal experimental outcomes.