Cross‐presentation of cell‐associated antigens by CD8α + dendritic cells is attributable to their ability to internalize dead cells

2002/10/01 by Oliver Schulz, Caetano Reis e Sousa

paper · doi:10.1046/j.1365-2567.2002.01513.x

crossref issued 2002/10/01 · crossref published 2002/10/01 · crossref published-print 2002/10/01 · crossref published-online 2002/10/16 · crossref created 2003/03/12 · crossref deposited 2023/10/17 · crossref indexed 2026/07/31

Abstract

Summary In the mouse, cross‐presentation is an exclusive property of the CD8α + subset of dendritic cells (DC) but the basis for this selectivity remains unclear. Here we report that splenic CD8α + DC are much superior to other DC subsets in internalizing dying cells in vitro . In contrast, CD8α + , CD8α – CD4 + and CD8α – CD4 – DC subsets phagocytose bacteria or latex beads to a similar extent. Although CD8α + DC are better than CD4 + DC at presenting ovalbumin (OVA)‐loaded splenocytes to naïve OT‐I T lymphocytes, CD4 + DC are better at presenting OVA‐expressing Escherichia coli to the same T cells. In both cases, presentation is abrogated by lactacystin. These results show that both splenic CD8α + and CD8α – DC can present exogenous antigens on major histocompatibility complex (MHC) class I via a proteasome‐dependent pathway and suggest that the specialized cross‐presenting function of CD8α + DC is a result of their ability to endocytose dying cells rather than a unique pathway for handling endosomal contents.

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