2026/04/10 by José Losa, François Simon, Dmitrii Linnik +16 · 1 voice
Biochemistry, Genetics and Molecular Biology · Engineering · #Protein Structure and Dynamics #Granular flow and fluidized beds #Yersinia bacterium, plague, ectoparasites research
paper · doi:10.1016/j.molcel.2026.03.018
Summary Molecular crowding in the bacterial cytoplasm restricts the diffusion of large molecules, impacting cellular processes. However, how nutrient availability influences cytoplasmic rheology is not well understood. With single-particle tracking in Escherichia coli , we observed a threefold variation in the diffusion of a 40-nm particle across exponential growth conditions. Previously suggested determinants of rheology did not account for this variation; instead, we found a strong anticorrelation between the diffusion coefficient and the abundance of amino acid metabolism proteins, persisting upon genetic perturbations and showing that lower diffusion is associated with increased viscoelasticity. Photoactivated light microscopy revealed that some amino acid metabolism proteins form clusters. Electron microscopy showed that these proteins could form amorphous agglomerates at physiological concentrations in vitro, likely driven by their low intrinsic disorder, high compactness and hydropathy score. These findings show that protein agglomerates regulate cytoplasmic rheology in a condition-dependent manner, suggesting an underappreciated level of cytoplasmic organization. Highlights Diffusion of 40-nm particles varies threefold across growth conditions in E. coli Cytoplasmic diffusion inversely correlates with COG-E protein abundance COG-E proteins form agglomerates that increase cytoplasmic viscoelasticity Protein compactness and hydrophobicity predict condition-dependent crowding effects