2026/02/16 by Huda M. Younis, Mohamed A. Abdel-Lateef, Amal A. Mohamed · 1 voice
Biochemistry, Genetics and Molecular Biology · Materials Science · Medicine · #Carbon and Quantum Dots Applications #Folate and B Vitamins Research #Sulfur Compounds in Biology
paper · doi:10.1177/00037028261427749
openalex publication_date 2026/02/16 · openalex created_date 2026/02/17 · openalex updated_date 2026/07/12
A sensitive, simple, inexpensive, rapid, and eco-friendly spectrofluorimetric method was created to assess the folic acid (FA) concentration in tablets using acriflavine (ACF) as an eco-friendly photoprobe. Based on its ability to quench the ACF fluorescence intensity in water at pH 8.0 and λ ex = 460nm. FA concentration was measured by quenching the fluorescence intensity of the ACF at λ em =508 nm within the linear range of 3.5 × 10 –6 –30.0 × 10 –6 mol L –1 with a correlation coefficient r 2 = 0.9991. The limit of quantification (LOQ) and the limit of detection (LOD) are 1.159 × 10 −6 mol·L –1 and 0.383 × 10 −6 mol·L –1 , respectively. This method was easy to use and accurately and effectively evaluated FA in pharmaceutical tablet samples. No influence was observed from the excipients usually contained in pharmaceutical formulations. The method was verified as valid according to the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) guidelines. In addition, its greenness has been estimated using environmental assessment tools.