2020/06/15 by The dMIQE Group, Alexandra S. Whale, Ward De Spiegelaere +31 · 1 voice · 3 citations
Biochemistry, Genetics and Molecular Biology · Engineering · #Biosensors and Analytical Detection #Innovative Microfluidic and Catalytic Techniques Innovation #Molecular Biology Techniques and Applications
paper · pdf · doi:10.1093/clinchem/hvaa125
openalex publication_date 2020/06/15 · openalex created_date 2020/08/10 · openalex updated_date 2026/07/28
Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an update to dMIQE, dMIQE2020, including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology.