Involvement of a Noncanonical Polyketide Synthase–Nonribosomal Peptide Synthetase Hybrid in the Biosynthesis of Sterol-C4-methyl Oxidase Inhibitor PF1163A

2024/12/24 by Qiaolin Ji, Xingxing Wei, Shike Liu +1 · 1 voice · 1 citation
Medicine · Agricultural and Biological Sciences · #Microbial Natural Products and Biosynthesis #Fungal Biology and Applications #Mycorrhizal Fungi and Plant Interactions

paper · pdf · doi:10.1021/acs.orglett.4c03675

openalex publication_date 2024/12/24 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/02

Abstract

High Resolution Image Download MS PowerPoint Slide PF1163A ( 1 ) is a fungal metabolite that inhibits sterol-C4-methyl oxidase. In this study, we identified the biosynthetic gene cluster of 1 and elucidated its biosynthetic pathway through heterologous expression experiments. Polyketide synthase–nonribosomal synthetase hybrid PfaA, which is responsible for the biosynthesis of PF1163A, harbors an unusual domain organization with tandem condensation (C) domains and a terminal condensation domain. Mutagenesis studies suggest that both C domains are required for the function of PfaA.

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• Fungal polyketide biosynthesis as a platform for designer natural products

Discussions

• One step #TotalSynthesis of PF1163A by the Matsuda group. doi.org/10.1021/acs.... [bsky, 2 points, 0 comments]

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