Elucidating the solution structure of the monomolecular BCL2 RNA G-quadruplex: a new robust NMR assignment approach

2025/01/01 by Zenghui Wang, Carla Ferreira Rodrigues, Simon Jurt +3 · 1 voice
Biochemistry, Genetics and Molecular Biology · #DNA and Nucleic Acid Chemistry #RNA Interference and Gene Delivery #RNA and protein synthesis mechanisms

paper · pdf · doi:10.1039/d5sc01416f

openalex publication_date 2025/01/01 · openalex created_date 2025/03/27 · openalex updated_date 2026/07/02

Abstract

proto-oncogene, one of the first natural monomolecular RNA G4 structures available to date. Comparative analysis with other RNA and DNA G4s reveals their notably compact and well-defined cores. Moreover, the sugar pucker geometries of the tetrad guanines are far less stringent than previously assumed, adeptly accommodating specific structural features. This contrasts with the canonical base pairing in RNA and DNA, in which the sugar pucker dictates the type of the double-helical structure. The strategy presented provides a direct path to uncovering G4 structural intricacies, advancing our grasp of their biological roles, and paving the way for RNA-targeted therapeutics.

Citations

• The regulation and functions of DNA and RNA G-quadruplexes

Discussions

• Do you want to assign solution structures of G-quadruplexes using NMR? Maybe this new approach we just published works for you. Congrats to Zhenghui and Carla for this amazing work! doi.org/10.1039/ [bsky, 2 points, 0 comments]

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