2025/03/01 by Jessica Labonté, Kathryn L. Campbell, Jordan R. Walker +1 · 1 voice · 1 citation
Environmental Science · #Methane Hydrates and Related Phenomena #Microbial Community Ecology and Physiology
paper · doi:10.1099/acmi.0.000865.v3
openalex publication_date 2025/03/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/26
dominated most of the very low biomass samples, including negative controls, indicating the order's ubiquity and its potential to be overamplified with common 16S rRNA amplification protocols. We amplified the 16S rRNA gene from preserved IODP legacy microbiological core samples and tested their level of contamination using the database. We also looked at published studies that did not sequence negative controls. Our results demonstrate that the type of drilling, amount of manipulation of the sample prior to preservation and sample depth, often associated with biomass, can influence the level of contamination within subsurface samples. This work provides an analysis framework for microbial taxonomic survey studies from low biomass subsurface samples for future scientific ocean drilling expeditions.