2025/04/17 by So-Young Lee, Hye-Min Woo, Hyunbum Jeon +10 · 1 voice
Medicine · Agricultural and Biological Sciences · #SARS-CoV-2 and COVID-19 Research #Animal Virus Infections Studies #Viral gastroenteritis research and epidemiology
paper · doi:10.1093/infdis/jiaf202
openalex publication_date 2025/04/17 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/22
The Middle East respiratory syndrome coronavirus (MERS-CoV) was first identified in 2012 and has since spread worldwide. To date, no vaccines or therapeutics against MERS-CoV have been approved for clinical use. The spike protein of MERS-CoV facilitates attachment and fusion with target cell membranes. Therefore, inhibiting S protein attachment represents a key therapeutic strategy for treating early MERS-CoV infection. Herein, we present 7 human neutralizing antibodies (KNIH-58, -68, -72, -78, -88, -90, and -95) against MERS-CoV. KNIH-58 and -68 bound to the receptor-binding subdomain (RBD) of the spike protein, while the other 5 monoclonal antibodies did not. KNIH-88, which targets the non-RBD region, exhibited potent neutralizing activities in vitro and in a transgenic mouse model, with similar results for KNIH-58. Structural analysis of KNIH-88 bound to the spike protein revealed novel epitopes in the non-RBD region. These findings may facilitate therapeutic and prophylactic antibody development against MERS-CoV.