2025/05/06 by Tim Van Den Bossche, Jean Armengaud, Dirk Benndorf +29 · 1 voice · 3 citations
Chemistry · Biochemistry, Genetics and Molecular Biology · #Advanced Proteomics Techniques and Applications #Genomics and Phylogenetic Studies #Metabolomics and Mass Spectrometry Studies
paper · pdf · doi:10.1002/imt2.70031
openalex publication_date 2025/05/06 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/31
Metaproteomics is an emerging approach for studying microbiomes, offering the ability to characterize proteins that underpin microbial functionality within diverse ecosystems. As the primary catalytic and structural components of microbiomes, proteins provide unique insights into the active processes and ecological roles of microbial communities. By integrating metaproteomics with other omics disciplines, researchers can gain a comprehensive understanding of microbial ecology, interactions, and functional dynamics. This review, developed by the Metaproteomics Initiative (www.metaproteomics.org), serves as a practical guide for both microbiome and proteomics researchers, presenting key principles, state-of-the-art methodologies, and analytical workflows essential to metaproteomics. Topics covered include experimental design, sample preparation, mass spectrometry techniques, data analysis strategies, and statistical approaches.