2023/08/26 by Erika Coletto, George M. Savva, Dimitrios Latousakis +7 · 1 voice
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · Nursing · #Gut microbiota and health #Infant Nutrition and Health #Probiotics and Fermented Foods
paper · pdf · doi:10.1038/s41598-023-40497-8
openalex publication_date 2023/08/26 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/31
Abstract Alterations in intestinal mucin glycosylation have been associated with increased intestinal permeability and sensitivity to inflammation and infection. Here, we used mice lacking core 3-derived O -glycans (C3GnT −/− ) to investigate the effect of impaired mucin glycosylation in the gut-brain axis. C3GnT −/− mice showed altered microbial metabolites in the caecum associated with brain function such as dimethylglycine and N -acetyl- l -tyrosine profiles as compared to C3GnT +/+ littermates. In the brain, polysialylated-neural cell adhesion molecule (PSA-NCAM)-positive granule cells showed an aberrant phenotype in the dentate gyrus of C3GnT −/− mice. This was accompanied by a trend towards decreased expression levels of PSA as well as ZO-1 and occludin as compared to C3GnT +/+ . Behavioural studies showed a decrease in the recognition memory of C3GnT −/− mice as compared to C3GnT +/+ mice. Combined, these results support the role of mucin O -glycosylation in the gut in potentially influencing brain function which may be facilitated by the passage of microbial metabolites through an impaired gut barrier.