2024/11/12 by Dominic Alcock, Sarahjane Power, Bridget Hogg +7 · 1 voice · 1 citation
Agricultural and Biological Sciences · Medicine · #Bat Biology and Ecology Studies #Viral Infections and Outbreaks Research #Viral Infections and Vectors
paper · pdf · doi:10.1038/s41598-024-76790-3
openalex publication_date 2024/11/12 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/23
Bats are becoming recognised as new model species to study naturally evolved mammalian extended healthspan and disease tolerance. However, this research is limited by the lack of bat specific cellular resources. Here we describe an optimised protocol to develop both primary and immortalised fibroblast cell-lines from wing biopsy punches from the Egyptian fruit bat, Rousettus aegyptiacus. We show that the immortalised cell lines and primary cells show similar characteristics in their proliferative capacity and response to oxidative stress. They also exhibited a similar response in their NF-κB immune response to TLR agonists including SARS-CoV2. As wing punches can be acquired non-lethally, these methods can be used to develop primary and immortalised cells, from potentially any bat species, including those of conservation concern that cannot be sacrificed. This can expand the scope of bat species that can be studied in the future, and the development of key cellular resources required to functionally validate the regulators of bats' unique longevity.