2025/06/06 by Paula Múgica-Galán, Jesús Miró-Bueno, Ángeles Hueso‐Gil +2 · 1 voice · 1 citation
Biochemistry, Genetics and Molecular Biology · Engineering · #Bacterial biofilms and quorum sensing #Biosensors and Analytical Detection #Innovative Microfluidic and Catalytic Techniques Innovation
paper · pdf · doi:10.1021/acssynbio.5c00036
openalex publication_date 2025/06/06 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
High Resolution Image Download MS PowerPoint Slide Engineering synthetic consortia to perform distributed functions requires robust quorum sensing (QS) systems to facilitate communication between cells. However, the current QS toolbox lacks standardized implementations, which are particularly valuable for use in bacteria beyond the model species Escherichia coli . We developed a set of three QS systems encompassing both sender and receiver modules, constructed using backbones from the SEVA (Standard European Vector Architecture) plasmid collection. This increases versatility, allowing plasmid features like the origin of replication or antibiotic marker to be easily swapped. The systems were characterized using the synthetic biology chassis Pseudomonas putida . We first tested individual modules, then combined sender and receiver modules in the same host, and finally assessed the performance across separate cells to evaluate consortia dynamics. Alongside the QS set, we provide mathematical models and rate parameters to support the design efforts. Together, these tools advance the engineering of robust and predictable multicellular functions.