2025/08/23 by Kai Yin, Zhijian Hu, Chuyan Chen +3 · 1 voice
Biochemistry, Genetics and Molecular Biology · Engineering · Agricultural and Biological Sciences · #Polyamine Metabolism and Applications #Biofuel production and bioconversion #Plant Disease Resistance and Genetics
paper · pdf · doi:10.1080/21501203.2025.2542561
openalex publication_date 2025/08/23 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/14
The basidiomycete Sporisorium scitamineum causes sugarcane smut disease, leading to a significant impairment in the quality and yield of sugarcane. Polyamines affect the intracellular redox balance, which is important for the pathogenic development of S. scitamineum, including sexual mating, dikaryotic hyphae growth, and host invasion. In the present study, we identified a polyamine transporter, SsTPO1, in S. scitamineum. Deletion or overexpression of SsTPO1 led to the disruption of intracellular levels of putrescine, spermidine, and spermine in opposite directions. SsTPO1-based polyamine homeostasis contributes to dikaryotic hyphal growth and host invasion after sexual mating, likely by affecting the cAMP-PKA signaling pathway and collaboratively regulating redox homeostasis. Environmental pH affected SsTPO1-mediated polyamine transport, so S. scitamineum hyphae growth favoured neutral to alkaline environments rather than acidic conditions. The SsTPO1-based polyamine transport also participates in stress resistance and autophagy induction. In summary, our work revealed that SsTPO1 facilitates polyamine transport to regulate cell growth, differentiation, and fungal pathogenicity in S. scitamineum.