2025/08/01 by Javier‐Fernando Montero‐Bullón, Javier Martín‐González, Gloria Muñoz‐Fernández +2 · 1 voice
Biochemistry, Genetics and Molecular Biology · Engineering · #Microbial Metabolic Engineering and Bioproduction #Biofuel production and bioconversion #Enzyme Catalysis and Immobilization
paper · pdf · doi:10.1111/1751-7915.70209
openalex publication_date 2025/08/01 · openalex created_date 2025/08/07 · openalex updated_date 2026/07/23
ABSTRACT The development of lipogenic biocatalysts with enhanced ability for agro‐waste bioconversion represents a critical challenge in microbial biotechnology, requiring the metabolic optimisation of different oil‐producing microorganisms. Ashbya gossypii , although not classified as an oleaginous microorganism, exhibits a significant capacity for intracellular lipid accumulation and can efficiently metabolise various low‐cost substrates, including xylose‐rich feedstocks. Indeed, previous works have shown metabolically engineered strains of A. gossypii reaching between 20% and 40% of the cell dry weight in lipid content when using xylose as the primary carbon source. Building on these findings, this study employs a multigenic optimisation strategy to further enhance lipid production by increasing the lipogenic supply of both acetyl‐CoA and NADPH, while simultaneously abolishing β‐oxidation, in a strain engineered for efficient xylose utilisation. The optimised strain achieved approximately 60% lipid content when cultivated on mixed sugar formulations designed to simulate xylose‐rich feedstocks. These results underscore the potential of A. gossypii as a promising microbial platform for sustainable lipid production from renewable resources, reinforcing its utility in biotechnological applications for bio‐based chemical and biofuel production.