Structural basis of SIRT2 pre-catalysis NAD + binding dynamics and mechanism

2025/01/01 by Nan Zhang, Kah Chee Pow, Lanfang Chen +1 · 1 voice
Medicine · #Sirtuins and Resveratrol in Medicine

paper · pdf · doi:10.1039/d5cb00169b

openalex publication_date 2025/01/01 · openalex created_date 2025/09/01 · openalex updated_date 2026/07/28

Abstract

In this paper, the structures of six different states (from pre-NAD + binding to intermediate I) during the SIRT2 deacylase reaction and two catalytically essential mutants were captured to elucidate their functional roles in the enzymatic mechanism.

Citations

• Phasercrystallographic software
• UCSF ChimeraX: Structure visualization for researchers, educators, and developers

Discussions

• New from Christopher Coxon et al., from @edinburgh-uni.bsky.social "19F NMR-tags for peptidyl prolyl conformation analysis" Fluorinated tags + 19F NMR = fresh insights into proline cis/trans isomeri [bsky, 2 points, 0 comments]

Related

• Identification of a novel small molecule that inhibits deacetylase but not defatty-acylase reaction catalysed by SIRT2
• Elucidating the Unconventional Binding Mode of a DNA‐Encoded Library Hit Provides a Blueprint for Sirtuin 6 Inhibitor Development
• The Sir2 Family of Protein Deacetylases
• Structural and enzymatic plasticity of SIRT6 deacylase activity
• Structural insights into the catalytic mechanism of the microcystin tailoring enzyme McyI