2025/09/09 by Adam P. Ryan, Cláudia Carvalho, Yiran Zhao +8 · 1 voice
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Mycorrhizal Fungi and Plant Interactions #Plant Pathogens and Fungal Diseases #Yeasts and Rust Fungi Studies
paper · doi:10.1099/ijsem.0.006898
openalex publication_date 2025/09/09 · openalex created_date 2025/09/10 · openalex updated_date 2026/07/02
Two yeast strains, PYCC 10015 and PYCC 10016, were isolated from soil from an Irish forest. Sequence analysis of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) of the rRNA gene repeat, and the D1/D2 domain of the LSU rRNA gene, showed that they belong to the Cyberlindnera and Barnettozyma genera of the order Phaffomycetales , but they did not exactly match any known species. The genomes of both isolates were sequenced using Oxford Nanopore Technologies and Illumina sequencing, generating chromosome-level genome assemblies. Phylogenomic analysis of 1,385 single-copy orthologues from 37 Phaffomycetales species and 2 outgroups showed that the closest relative of PYCC 10015 is Cyberlindnera galapagoensis and that PYCC 10016 is placed in a subclade containing 7 other species from the Barnettozyma genus. The average nucleotide identity between these strains and their closest relatives is <75%, supporting their designation as novel species. Here, we propose the names Cyberlindnera hibernica sp. nov. and Barnettozyma discipulorum sp. nov. for PYCC 10015 and PYCC 10016, respectively.