2026/07/13 by Shou-Mian Li, Fei Liu, Guojie Li +5 · 1 voice
Medicine · Agricultural and Biological Sciences · #Fungal Biology and Applications #Silymarin and Mushroom Poisoning #Mycorrhizal Fungi and Plant Interactions
paper · doi:10.1080/21501203.2026.2693413
openalex publication_date 2026/07/13 · openalex created_date 2026/07/14 · openalex updated_date 2026/07/16
Grifola albicans f. huishuhua (formerly treated as Grifola frondosa, Maitake) is an economically important edible and medicinal mushroom whose breeding is constrained by limited genetic traceability and insufficient molecular markers. Here, we generated a high-quality reference genome for strain YS74 and analyzed 101 wild and cultivated strains from diverse geographic origins. Using 83 conserved single-copy core genes, we identified 2551 high-confidence SNPs and developed a core gene-associated multiple nucleotide polymorphism (cgMNP) framework. Population structure and phylogenetic analyses consistently resolved four major genetic clusters, reflecting differentiation associated with domestication history and regional breeding practices. Developmental and agronomic traits varied among clusters, with prolonged developmental cycles generally associated with reduced harvest duration. Among the core genes, Hsp70 contained the largest number of detected SNPs and largely recapitulated the relationships inferred from the genome-wide dataset. These results demonstrate that cgMNP markers provide a robust and scalable approach for strain discrimination, population analysis, and germplasm characterization, while offering new insights into the genetic diversity and breeding history of G. albicans f. huishuhua.