2024/12/01 by Diyana S. Sokolova, D. Sh. Sokolova, T. L. Babich +9
Engineering · Environmental Science · #Enhanced Oil Recovery Techniques #Hydrocarbon exploration and reservoir analysis #Microbial bioremediation and biosurfactants
paper · doi:10.1134/s0026261724609631
crossref issued 2024/12/01 · crossref published 2024/12/01 · crossref published-print 2024/12/01 · openalex publication_date 2024/12/01 · crossref published-online 2025/02/27 · crossref created 2025/02/27 · openalex created_date 2025/10/10 · crossref deposited 2026/03/29 · crossref indexed 2026/07/29 · openalex updated_date 2026/07/30
Abstract— Oil reservoirs with carbonate oil-bearing collectors are characterized by high fracturing. To improve the coverage of a reservoir by flooding, clogging agents should be introduced into the formation. In the patent literature, using sapropel as a plugging agent has been proposed. In the present work, application of cultural methods revealed that sapropel can be not only a dispersant, but also a source of aerobic hydrocarbon-oxidizing and anaerobic fermenting bacteria. Taxonomic analysis of the sapropel microbial community using high-throughput sequencing of the 16S rRNA gene V3–V4 region showed predominance of bacteria of the genus Streptomyces (40.7%) of the phylum Actinomycetota (55% of the total number of sequences). The possibility of using starch, sucrose, and other sugars by the microorganisms of sapropel and reservoir water predicted using the iVikodak program was also confirmed experimentally. Formation microbiota and sapropel bacteria fermented starch and sucrose (a molasses component) with formation of alcohols and lower fatty acids, which may contribute in decreasing the viscosity of oil, dissolve rock carbonates and lead to enhanced oil recovery.