2024/08/01 by Yu. V. Zeleneva, И. Б. Аблова, L. M. Mokhova · 1 voice
Agricultural and Biological Sciences · #Wheat and Barley Genetics and Pathology #Plant Disease Resistance and Genetics #Mycotoxins in Agriculture and Food
paper · doi:10.3103/s1068367424700393
openalex publication_date 2024/08/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/31
Abstract The study was aimed at clarifying species composition of wheat Septoria pathogens and characterizing populations of Parastagonospora nodorum and P. pseudonodorum for the presence of Tox1, Tox3, ToxA, and Tox267 effector genes that is important for providing artificial infection background and identifying the sources and donors of wheat resistance to Septoria leaf blotch diseases. The study was carried out in Krasnodar krai and Leningrad oblast using samples of infected plants collected in 2023. The dominating species, Zymoseptoria tritici, was revealed in all plant samples (100% occurrence). P. nodorum and P. pseudonodorum were observed less frequently; their occurrence in Leningrad oblast was 80 and 60%, respectively, whereas it was 11.76 and 35.29%, respectively, for Krasnodar krai. The presence of the Tox267 gene in the studied Russian populations of Parastagonospora spp. was first examined using the SnTox2DONRF/SnTox2DONRS primer pair. The performed molecular screening revealed the ToxA and Tox1 genes in both P. nodorum and P. pseudonodorum, while the Tox3 and Tox267 genes were identified only in P. nodorum. Among 28 studied P. nodorum isolates, eight isolates from Krasnodar krai (29%) contained the ToxA gene. The Tox1 gene was revealed in five isolates from Leningrad oblast and four isolated from Krasnodar krai (32%). The Tox3 gene was identified in eight isolates from Krasnodar krai and ten isolated from Leningrad oblast (64%). The Tox267 gene was found only in the genotype of eight isolates from Krasnodar krai, i.e., in 29% of the studied isolates. Among 87 isolates of P. pseudonodorum, the ToxA gene was found in 5% of genotypes (four isolates from Krasnodar krai), while the Tox1 gene was identified in 23% of isolates (17 isolates from Leningrad oblast and three isolates from Krasnodar krai).