2026/03/31 by C. Chen, Qiang Zhang, Yonggang Xu +8 · 1 voice
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Aluminum toxicity and tolerance in plants and animals #Mechanisms of cancer metastasis #Plant Stress Responses and Tolerance
paper · doi:10.1111/pbi.70638
openalex publication_date 2026/03/31 · openalex created_date 2026/04/01 · openalex updated_date 2026/07/27
-transcription factor OsART1 plays a crucial role in regulating Al tolerance in rice by modulating the expression of specific genes. However, the posttranscriptional regulation of OsART1 remains poorly understood. In this study, we identified and characterised OsWRKY64, which interacts with OsART1. OsWRKY64 was localised in the nucleus. Tissue expression analysis indicated that OsWRKY64 was primarily expressed in the roots, and its transcription and protein accumulation were significantly induced by Al. The oswrky64 mutants and the OsWRKY64 overexpression lines exhibited decreased or increased expression of genes regulated by OsART1, which consequently led to phenotypes sensitive or resistant to Al, respectively. Further analysis indicated that OsWRKY64 interacted with OsART1 to promote its stability, thereby accelerating the transcriptional activity of OsART1 on its downstream genes. Additionally, OsART1 upregulated OsWRKY64 expression by directly binding to the OsWRKY64 promoter, thus forming a negative feedback loop between OsART1 and OsWRKY64. Overall, our results demonstrated that OsWRKY64 influences Al resistance by interacting with and stabilising OsART1 to accelerate its transcriptional activity.