2026/06/01 by Eli Slenders, Eleonora Perego, Sabrina Zappone +1 · 1 voice
Biochemistry, Genetics and Molecular Biology · Chemistry · Medicine · #Advanced Fluorescence Microscopy Techniques #Optical Imaging and Spectroscopy Techniques #Photochemistry and Electron Transfer Studies
paper · pdf · doi:10.1088/2515-7647/ae769d
openalex publication_date 2026/06/01 · openalex created_date 2026/06/03 · openalex updated_date 2026/07/22
Abstract Fluorescence fluctuation spectroscopy (FFS) is an ensemble of techniques for the quantitative measurement of molecular dynamics and interactions. Recently, the introduction of small-format array detectors has opened up a new range of spatiotemporal information, enabling more detailed analysis of system kinetics. However, there is currently no open-source software available for analyzing the high-dimensional FFS data sets. We present BrightEyes-FFS, an open-source Python-based environment for FFS analysis with array detectors. The environment includes a Python package for reading raw FFS data, computing auto- and cross-correlations using various algorithms, and fitting the correlations to several models. A graphical user interface (GUI), available as a standalone executable, makes the analysis fast and user-friendly. An automated Jupyter Notebook writing tool enables users to transfer GUI-based analyses to Jupyter Notebook for custom analysis. We believe that BrightEyes-FFS will enable a wider community to study diffusion, flow, and interaction dynamics.