2024/01/26 by Lei Jin, Ziyang Yu, Aaron Au +5 · 1 voice
Engineering · Physics and Astronomy · #Advanced Optical Imaging Technologies #Digital Holography and Microscopy #Image Processing Techniques and Applications
paper · doi:10.1016/j.ohx.2024.e00508
openalex publication_date 2024/01/26 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/29
We present the design of a low-cost, portable telecentric digital holographic microscope (P-TDHM) that utilizes off-the-shelf components. We describe the system's hardware and software elements and evaluate its performance by imaging samples ranging from nano-printed targets to live HeLa cells, HEK293 cells, and Dolichospermum via both in-line and off-axis modes. Our results demonstrate that the system can acquire high quality quantitative phase images with nanometer axial and sub-micron lateral resolution in a small form factor, making it a promising candidate for resource-limited settings and remote locations. Our design represents a significant step forward in making telecentric digital holographic microscopy accessible and affordable to the broader community.