2015/02/28 by Jason C. Kwong, J.C. Kwong, Nadine McCallum +5 · 4 citations
Biochemistry, Genetics and Molecular Biology · Medicine · #Bacterial Identification and Susceptibility Testing #Genomics and Phylogenetic Studies #Mycobacterium research and diagnosis
paper · doi:10.1097/pat.0000000000000235
openalex publication_date 2015/02/28 · crossref created 2015/02/28 · crossref issued 2015/04/01 · crossref published 2015/04/01 · crossref published-print 2015/04/01 · crossref deposited 2018/09/24 · openalex created_date 2025/10/10 · crossref indexed 2026/07/28 · openalex updated_date 2026/08/01
Genomics and whole genome sequencing (WGS) have the capacity to greatly enhance knowledge and understanding of infectious diseases and clinical microbiology.The growth and availability of bench-top WGS analysers has facilitated the feasibility of genomics in clinical and public health microbiology.Given current resource and infrastructure limitations, WGS is most applicable to use in public health laboratories, reference laboratories, and hospital infection control-affiliated laboratories.As WGS represents the pinnacle for strain characterisation and epidemiological analyses, it is likely to replace traditional typing methods, resistance gene detection and other sequence-based investigations (e.g., 16S rDNA PCR) in the near future.Although genomic technologies are rapidly evolving, widespread implementation in clinical and public health microbiology laboratories is limited by the need for effective semi-automated pipelines, standardised quality control and data interpretation, bioinformatics expertise, and infrastructure.