2002/10/01 by Erica Spackman, E. Spackman, S. S. Cloud +2
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Animal Virus Infections Studies #Plant Virus Research Studies #Virus-based gene therapy research
paper · doi:10.1637/0005-2086(2002)046[0956:coapss]2.0.co;2
crossref issued 2002/10/01 · crossref published 2002/10/01 · crossref published-print 2002/10/01 · openalex publication_date 2002/10/01 · crossref created 2006/07/12 · crossref deposited 2019/04/20 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/26 · crossref indexed 2026/07/25
A putative new serotype of chicken infectious anemia virus (CIAV) isolated from 17-wk-old broiler breeder pullets was compared with a known, previously characterized CIAV isolate, the Del-Ros strain. Physicochemical characteristics evaluated induded thermal stability, size, pH, and chloroform sensitivity. Physicochemically, CIAV-7 was identical to CIAV. The virus isolates were compared antigenically by enzyme-linked immunosorbent assay, virus neutralization, immunofluorescence assay, and western blot. All four serologic assays demonstrated that CIAV-7 is antigenically distinct from the Del-Ros strain of CIAV. Additionally, polymerase chain reaction (PCR) and Southern blot were used to determine if there were similarities in genome sequence between the two viruses. CIAV-7 could not be detected with CIAV-specific PCR primers or a with CIAV-specific probe by Southern hybridization.