2024/12/11 by Shilpa Saseendran Nair, Torsten Kleffmann, Briana Smith +4 · 1 voice
Biochemistry, Genetics and Molecular Biology · Medicine · #Bacterial Identification and Susceptibility Testing #Antimicrobial Resistance in Staphylococcus #Bacterial biofilms and quorum sensing
paper · doi:10.1016/j.ab.2024.115746
openalex publication_date 2024/12/11 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/30
Staphylococcus aureus is a significant human pathogen causing acute life-threatening, and chronic infections often linked to biofilms. This study conducted a comparative lipidomic analysis of a methicillin-resistant (MRSA) and a methicillin-susceptible (MSSA) S. aureus strain in both planktonic and biofilm cultures using liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) spectroscopy. The developed protocol successfully differentiates between the strains in various living states (planktonic and biofilm) and growth media (Tryptic Soy Broth and Brain Heart Infusion) using S. aureus USA300 LAC (MRSA) and S. aureus Newman (MSSA). LC-MS and NMR lipidomics profiles revealed global differences and particular ones among the following classes of bacterial lipids: phosphatidylglycerols, diacylglycerols, monoglycosyldiacylglycerols, diglycosyldiacylglycerols, and cardiolipins. Lipid content was higher in the biofilm states for most of these classes. Growth media differences were significant, while differences between MRSA and MSSA were less pronounced but still detectable. Additionally, we provide data on hundreds of unknown compounds that differ based on living state, strain background, or growth media. This study offer insights into the dynamic nature of S. aureus lipid composition and the used methods are adaptable to other organisms. • Bioanalytical lipidomics investigations of bacterial samples using LC-MS and NMR). • Comparing MRSA and a MSSA S. aureus strain samples using these two techniques in tandem. • Growth media background, as well as living state influences the lipid profile of bacterial samples. • Increase in lipid content in the biofilm state. • Specific lipid molecules increase or decrease between living states or between strains.