Using environment-sensitive tetramethylated thiophene-BODIPY fluorophores in DNA probes for studying effector-induced conformational changes of protein–DNA complexes

2025/01/01 by Markéta Šoltysová, Pedro Güixens‐Gallardo, Irena Sieglová +7 · 2 voices
Biochemistry, Genetics and Molecular Biology · #DNA and Nucleic Acid Chemistry #RNA Interference and Gene Delivery #RNA and protein synthesis mechanisms

paper · pdf · doi:10.1039/d4cb00260a

openalex publication_date 2025/01/01 · openalex created_date 2025/01/02 · openalex updated_date 2026/08/01

Abstract

Using fluorophore-linked probes, we revealed conformational changes affecting protein–DNA affinity that were undetectable using traditional methods. We identified l -lactate as the effector molecule of transcriptional repressor LutR in B. subtilis .

Citations

• Genetic regulatory mechanisms in the synthesis of proteins
• Highly accurate protein structure prediction with AlphaFold
• Structural characterization of two prototypical repressors of SorC family reveals tetrameric assemblies on DNA and mechanism of function

Discussions

• #research Using environment-sensitive tetramethylated thiophene-BODIPY fluorophores in DNA probes for studying effector-induced conformational changes of protein–DNA complexes (@hoceklab.bsky.social [bsky, 3 points, 0 comments]
• Our new paper (collaboration with P. Rezacova group) on the use of environment-sensitive fluorophore-linked nucleotide in DNA probes for identification of effectors of TFs and studying conformational [bsky, 2 points, 0 comments]

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