2025/05/01 by Caijin Chen, Panthita Ruang‐areerate, Anthony J. Travis +6 · 1 voice
Agricultural and Biological Sciences · Environmental Science · #Aluminum toxicity and tolerance in plants and animals #Arsenic contamination and mitigation #Rice Cultivation and Yield Improvement
paper · pdf · doi:10.1002/pld3.70064
openalex publication_date 2025/05/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/23
ABSTRACT Rice is a globally important crop and is particularly efficient at assimilating arsenic (As). Identifying QTLs and genes associated with grain As is essential for breeding low‐As rice cultivars. In this study, data on As accumulation in grains of Rice Diversity Panel 1 in five field environments at four diverse geographic sites were reanalyzed to compare genome‐wide association (GWA) methods. Two single‐locus (EMMAX for single trait and GEMMA for multi‐experiments) and six multi‐locus (FASTmrEMMA, ISIS EM‐BLASSO, mrMLM, pKWmEB, pLARmEB, and FASTmrMLM) GWA methods were used. A total of 90 and 111 QTLs were detected using EMMAX and GEMMA, respectively. A total of 2, 11, 12, 19, 23, and 25 QTNs were identified by FASTmrEMMA, ISIS EM‐BLASSO, mrMLM, pKWmEB, pLARmEB, and FASTmrMLM, respectively. Among these, 22 QTLs/QTNs were co‐detected by single‐locus and multi‐locus GWAS methods. From these QTLs/QTNs, a total of 10 candidate genes were identified. Analysis of the haplotype variants of one candidate genes, OsABCC1 , and one cluster of the plasma membrane intrinsic proteins genes revealed that a greater than 10% reduction in grain As could be achieved. The QTLs/QTNs and candidate genes identified give insight into the molecular mechanisms regulating As accumulation in rice and serve as breeding targets for developing low grain As rice cultivars.