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Trends in Direct Breath Analysis by Secondary Electrospray Ionization Mass Spectrometry for Clinical Applications

2022/04/27 by Bettina Streckenbach
Biochemistry, Genetics and Molecular Biology · Chemistry · Engineering · #Advanced Chemical Sensor Technologies #Mass Spectrometry Techniques and Applications #Metabolomics and Mass Spectrometry Studies

paper · pdf · doi:10.2533/chimia.2022.322

crossref issued 2022/04/27 · crossref published 2022/04/27 · crossref published-online 2022/04/27 · openalex publication_date 2022/04/27 · crossref created 2022/04/27 · crossref deposited 2022/09/19 · openalex created_date 2025/10/10 · crossref indexed 2026/07/24 · openalex updated_date 2026/07/25

Abstract

Exhaled breath can reveal insights about the metabolic state of the human body through the endo- and exogenous compounds it contains. The extent of detectable compounds, however, was revolutionized by the application of mass spectrometry. More specifically, secondary electrospray ionization high-resolution mass spectrometry (SESI-HRMS) enables the detection of a broad range of breath-derived compounds simultaneously and with high sensitivity. Together with its rapid and non-invasive nature, direct breath analysis by SESI-HRMS raised particular interest for clinical applications. Over the past years, various clinical trials successfully demonstrated the technology´s capability for biomarker discovery in exhaled breath in adults and more recently in children. Current challenges lie within the potential translation of SESI-HRMS into clinical settings and the requirements therein, such as biomarker identification and validation, which became a focus of more recent studies.

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