2022/02/23 by Serge Rudaz, Elena Tobolkina, Víctor González-Ruiz +6
Biochemistry, Genetics and Molecular Biology · Chemistry · #Advanced Proteomics Techniques and Applications #Mass Spectrometry Techniques and Applications #Metabolomics and Mass Spectrometry Studies
paper · pdf · doi:10.2533/chimia.2022.90
crossref issued 2022/02/23 · crossref published 2022/02/23 · crossref published-online 2022/02/23 · openalex publication_date 2022/02/23 · crossref created 2022/02/23 · openalex created_date 2022/03/02 · crossref deposited 2022/09/19 · crossref indexed 2026/07/28 · openalex updated_date 2026/07/28
Untargeted metabolomics is now widely recognized as a useful tool for exploring metabolic changes taking place in biological systems under different conditions. In this article, we aim to provide a short overview of the liquid-phase separation methods hyphenated to MS to perform untargeted metabolomics of biological samples. Each approach is complemented by up-to-date literature to guide readers, as well as practical information for avoiding or fixing some of the most frequently encountered pitfalls. This article covers mainly data acquisition, but a short discussion is provided regarding signal processing and data treatment, as well as data analysis and its biological interpretation in the context of metabolomic studies.