2026/07/29 by Qiuping Wang, Dongsheng Chen, Ting Li
Biochemistry, Genetics and Molecular Biology · Immunology and Microbiology · #Atherosclerosis and Cardiovascular Diseases #MicroRNA in disease regulation #RNA regulation and disease
paper · doi:10.1177/00033197261471988
crossref issued 2026/07/29 · crossref published 2026/07/29 · crossref published-online 2026/07/29 · openalex publication_date 2026/07/29 · crossref created 2026/07/29 · crossref deposited 2026/07/29 · crossref indexed 2026/07/29 · openalex created_date 2026/07/30 · openalex updated_date 2026/07/30
The progression of acute coronary syndrome (ACS) is primarily determined by the transition from stable to vulnerable atherosclerotic plaque. The primary objective of this study was to investigate the mechanism of SLC25A25 antisense RNA 1 (SLC25A25-AS1) in ACS. The level of SLC25A25-AS1 was determined using reverse transcription quantitative real-time PCR (RT-qPCR) in serum samples from healthy controls (n = 81) and ACS patients (n = 162). The clinical relevance of SLC25A25-AS1 in ACS was evaluated through correlation, receiver operating characteristic (ROC), and logistic regression analyses. SLC25A25-AS1 was significantly downregulated in ACS patients. Overexpression of SLC25A25-AS1 protected oxidized low-density lipoprotein (ox-LDL) treated human coronary artery smooth muscle cells (HCASMCs) from damage in an in vitro atherosclerosis model. Specifically, these protective effects were against abnormal cell proliferation, inflammatory responses, cell migration, and maintenance of the contractile phenotype in HCASMCs. Both bioinformatics analyses and experimental validation confirmed the existence of the SLC25A25-AS1/ microRNA‑34a‑5p (miR-34a-5p)/Forkhead box P1 (FOXP1) regulatory axis. Collectively, SLC25A25-AS1 modulated ox-LDL-induced atherosclerotic responses in HCASMCs through the miR-34a-5p/FOXP1 pathway. These findings suggested that SLC25A25-AS1 may play a protective role in ACS by alleviating ox-LDL-induced HCASMC dysfunction and inflammation via the miR-34a-5p/FOXP1 axis.