2025/06/25 by Ian Coghill, Coghill, Ian, Aliénor Lahlou +11 · 1 voice
Biochemistry, Genetics and Molecular Biology · #Advanced Fluorescence Microscopy Techniques
paper · doi:10.1364/oe.570450
openalex publication_date 2025/08/13 · openalex created_date 2025/08/17 · openalex updated_date 2026/07/28
Luminescence imaging is invaluable for studying biological and material systems, particularly when advanced protocols that exploit temporal dynamics are employed. However, implementing such protocols often requires custom instrumentation, either modified commercial systems or fully bespoke setups, which poses a barrier for researchers without expertise in optics, electronics, or software. To address this, we present a versatile macroscopic fluorescence imaging system capable of supporting a wide range of protocols, and provide detailed build instructions along with open-source software to enable replication with minimal prior experience. We demonstrate its broad utility through applications to plants, reversibly photoswitchable fluorescent proteins, and optoelectronic devices.