2025/06/04 by Suk Min Kim, So Yeon Kong, Jin-Gu Kang +7 · 1 voice
Biochemistry, Genetics and Molecular Biology · Energy · Environmental Science · #Metalloenzymes and iron-sulfur proteins #Microbial Fuel Cells and Bioremediation #Porphyrin Metabolism and Disorders
paper · pdf · doi:10.1002/anie.202508565
openalex publication_date 2025/06/04 · openalex created_date 2025/06/05 · openalex updated_date 2026/06/26
Abstract The inherent O 2 sensitivity of Ni─Fe carbon monoxide dehydrogenases (CODHs), crucial for rapid CO to CO 2 interconversion, presents substantial challenges for industrial application. Transforming CO/CO 2 , a prevalent anthropogenic air pollutant, into valuable carbon chemicals either directly or through intermediate steps via biocatalytic methods offers a promising pathway to achieve net‐zero emissions across industries and the environment. However, completely eliminating oxygen from industrial biotransformations, especially under ambient conditions, is exceedingly onerous. Here, we engineered variants of the CODH2 from Carboxydothermus hydrogenoformans ( Ch CODH2) with dual blocking at both the O 2 entrance and near the active site, effectively sealing the tunnel against atmospheric O 2 levels (20%). The O 2 ‐tunnel engineered A559W/V610H variant demonstrated a marked improvement in air stability, with a half‐life of 24.6 h compared to the wild type's 2.4 h. Crystallographic snapshots of this air‐viable variant after 24 h of exposure revealed the robust integrity of the fortified FeS and NiFeS clusters. Additionally, electro‐enzymatic reactions corroborated its CO/CO 2 conversion capability even in ubiquitous air. These findings, which address the O 2 sensitivity of anaerobic enzymes caused by O 2 ‐induced metal cluster collapse, enhance their potential for biological CO/CO 2 transformations in O 2 ‐rich environments, thereby broadening their industrial viability and applicability.