2026/07/07 by Manuela Agudelo-Restrepo, Patrick Dibouloul, Karen Harms +5 · 1 voice
Agricultural and Biological Sciences · Chemistry · Medicine · #Advanced Synthetic Organic Chemistry #Microbial Natural Products and Biosynthesis #Plant and fungal interactions
paper · pdf · doi:10.3897/mycokeys.136.191424
openalex publication_date 2026/07/07 · openalex created_date 2026/07/08 · openalex updated_date 2026/08/01
A decahydrofluorene-class alkaloid, pyrrospirone Z ( 1a ), along with pyrrospirone F ( 2 ), pyrrospirone M ( 3 ), GKK1032A2 ( 4 ), daldinol ( 5 ), orthosporin ( 6 ), and 3,4-dihydro-3,4,8-trihydroxy-1(2H)-naphthalenone ( 7 ), was purified from the new coprophilous species Schizothecium keniense , isolated from dung in Kenya. Structural elucidation was achieved using comprehensive 1D and 2D NMR spectroscopy, and high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), with relative configurations assigned based on NOESY correlations. Among the compounds, pyrrospirone F ( 2 ) and GKK1032A2 ( 4 ) displayed significant antibacterial activity against the Gram-positive bacteria Bacillus subtilis (MIC = 4.2 μg/mL and 8.3 μg/mL, respectively) and Staphylococcus aureus (MIC = 8.3 μg/mL for both). Additionally, compounds 2 and 4 showed mild antifungal activity against the filamentous fungi Mucor hiemalis at 33.3 μg/mL. Cytotoxicity assays revealed that GKK1032A2 ( 4 ) was the most potent compound, with IC 50 values ranging from 2.0 µM to 8.1 µM across the tested human cancer cell lines. This work represents the first investigation of the chemical constituents of the genus Schizothecium and underscores the potential of coprophilous fungi as valuable sources of biologically active metabolites.