2003/03/01 by Tesfaye Wubet, Michael Weiß, Ingrid Kottke +1
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Lichen and fungal ecology #Mycorrhizal Fungi and Plant Interactions #Plant Pathogens and Fungal Diseases
paper · doi:10.1139/b03-020
crossref issued 2003/03/01 · crossref published 2003/03/01 · crossref published-print 2003/03/01 · openalex publication_date 2003/03/01 · crossref created 2003/04/11 · crossref deposited 2020/03/18 · openalex created_date 2025/10/10 · crossref indexed 2026/07/29 · openalex updated_date 2026/07/29
Taxus baccata L. roots collected from two sites in southern Germany were heavily colonized by arbuscular mycorrhizal fungi (AMF). The colonization pattern was of the Paris type. The diversity of the colonizing AMF species was investigated using polymerase chain reaction based molecular techniques. The internal transcribed spacer (ITS) region of the DNA from AMF within the roots was amplified using Glomeromycota-specific primers and then cloned and sequenced. Phylogenetic analysis using a data set of 5.8S rDNA sequences from a wide range of glomeralean taxa as well as data sets of partial ITS2 sequences from glomeralean subgroups indicated root colonization by four sequence types of Glomus and one sequence type of Archaeospora. These sequence types are distinct from any previously published sequences and differed between the two study sites.Key words: arbuscular mycorrhiza, Paris-type AM, molecular diversity, ribosomal internal transcribed spacers, Taxus baccata.