2023/01/01 by Tatyana S. Kalebina, Ekaterina V. Kulakovskaya, Ekaterina Kulakovskaya +11
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · Medicine · #Coagulation, Bradykinin, Polyphosphates, and Angioedema #Fungal and yeast genetics research #Polysaccharides and Plant Cell Walls
paper · doi:10.1134/s0006297923010133
crossref issued 2023/01/01 · crossref published 2023/01/01 · crossref published-print 2023/01/01 · openalex publication_date 2023/01/01 · crossref published-online 2023/02/17 · crossref created 2023/02/17 · openalex created_date 2025/10/10 · crossref deposited 2026/03/16 · crossref indexed 2026/07/30 · openalex updated_date 2026/07/30
Inorganic polyphosphates (polyP), according to literature data, are involved in the regulatory processes of molecular complex of the Saccharomyces cerevisiae cell wall (CW). The aim of the work was to reveal relationship between polyP, acid phosphatase Pho3p, and the major CW protein, glucanosyltransglycosylase Bgl2p, which is the main glucan-remodelling enzyme with amyloid properties. It has been shown that the yeast cells with deletion of the PHO3 gene contain more high molecular alkali-soluble polyP and are also more resistant to exposure to alkali and manganese ions compared to the wild type strain. This suggests that Pho3p is responsible for hydrolysis of the high molecular polyP on the surface of yeast cells, and these polyP belong to the stress resistance factors. The S. cerevisiae strain with deletion of the BGL2 gene is similar to the Δpho3 strain both in the level of high molecular alkali-soluble polyP and in the increased resistance to alkali and manganese. Comparative analysis of the CW proteins demonstrated correlation between the extractability of the acid phosphatase and Bgl2p, and also revealed a change in the mode of Bgl2p attachment to the CW of the strain lacking Pho3p. It has been suggested that Bgl2p and Pho3p are able to form a metabolon or its parts that connects biogenesis of the main structural polymer of the CW, glucan, and catabolism of an important regulatory polymer, polyphosphates.