De novo assembly and annotation of the Empoasca fabae mitochondrial genome

2025/05/05 by Joshua Molligan, Jordanne Jacques, Soham Mukhopadhyay +1 · 1 voice · 2 citations
Biochemistry, Genetics and Molecular Biology · Environmental Science · #Genetic diversity and population structure #Genomics and Phylogenetic Studies #Microbial Community Ecology and Physiology

paper · doi:10.1080/23802359.2025.2498740

openalex publication_date 2025/05/05 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/15

Abstract

This study presents the assembly and annotation of the full-length mitochondrial genome for the leafhopper species Empoasca fabae Harris, 1841. The mitogenome was obtained from a contig-level assembly with the identified mitochondrial genome being 14,873 bp in length. The base composition was A (38.8%), T (39.1%), C (11.7%), and G (10.4%). The mitogenome comprised 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and showed a unique, non-AT-rich D-loop region. Phylogenetic analysis confirmed the placement of E. fabae within the subfamily Typhlocybinae, clustering with other species in the Empoasca genus.

Citations

• Basic local alignment search tool
• Using SPAdes De Novo Assembler
• Phylogeny of the tribe Empoascini (Hemiptera: Cicadellidae: Typhlocybinae ) based on morphological characteristics, with reclassification of the Empoasca generic group
• Interactive Tree Of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation
• IQ-TREE: A Fast and Effective Stochastic Algorithm for Estimating Maximum-Likelihood Phylogenies
• MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability
• Leafhoppers as markers of the impact of climate change on agriculture

Cited by

• Genome-resolved metagenomics reveals conserved, flexible and emerging symbioses across global leafhoppers
• Phylogenetic Analysis of Scaphoideus Reveals New Insights Into the Invasion History of Scaphoideus titanus (Hemiptera, Cicadellidae) in Europe

Discussions

• 📢 New Publication Alert! Excited to share our latest manuscript presenting the first genomic evidence of divergence between New World and Old World species within the genus Empoasca. Bravo Joshua � [bsky, 4 points, 0 comments]

Related

• De novo assembly and annotation of the Empoasca fabae mitochondrial genome
• A Chromosome-level Genome Assembly of the Potato Leafhopper Empoasca fabae (Hemiptera: Cicadellidae)
• Insect Mitochondrial Genomics: Implications for Evolution and Phylogeny
• Insect Mitochondrial Genomics: A Decade of Progress
• The genome sequence of the Stripe-backed Dasysyrphus, Dasysyrphus albostriatus (Fallén, 1817)