2025/09/23 by Tobias Greisle, Ines Kunze, Xianming Wang +4 · 1 voice
Biochemistry, Genetics and Molecular Biology · Chemistry · Medicine · #Click Chemistry and Applications #Monoclonal and Polyclonal Antibodies Research #Receptor Mechanisms and Signaling
paper · doi:10.1016/j.scr.2025.103838
openalex publication_date 2025/09/23 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/23
Deriving functional β-cells from human induced pluripotent stem cells (hiPSCs) holds potential for cell replacement therapy, disease modeling, and drug testing in diabetes research. Wnt/Planar cell polarity (PCP) signaling is crucial for endocrine cell development and β-cell maturation in murine models and can be tracked by the expression of the tissue-specific effector gene Flattop. Here, we report the generation of a human fluorescent FLTP/CFAP126 (Flattop-T2A-H2B-Venus) and FLTP-Insulin (Flattop-T2A-H2B-Venus x C-peptide-mCherry) double reporter by CRISPR/Cas9 gene editing. These hiPSC reporter lines allow monitoring of WNT/PCP signaling during endocrine cell formation and studying its role in β-cells in a human model system.