2026/06/15 by Kaoru Sugimura, Ryu Takayanagi, T. Namba +2 · 3 voices
Biochemistry, Genetics and Molecular Biology · #Cellular Mechanics and Interactions #Developmental Biology and Gene Regulation #Hippo pathway signaling and YAP/TAZ
paper · pdf · doi:10.1242/dev.205474
openalex publication_date 2026/06/15 · openalex created_date 2026/07/01 · openalex updated_date 2026/07/22
During morphogenesis, cell divisions are precisely regulated in space and time. The biological objectives achieved by such regulation are not fully understood. Here, by applying a newly developed lineage-reconstruction pipeline to Drosophila pupal wing, we reveal that the wing is composed of distinct cell groups that differ in division number, timing, and spatial positioning relative to wing veins. We show that the frequencies of these lineages, together with their initial cell sizes and growth profiles, converge to achieve a highly conserved average cell size. Our data further suggest that distance from veins provides spatial information that biases where distinct lineages arise, and that loss of veins caused by perturbation of EGFR signaling suppresses a specific lineage and disrupts cell-size control. Finally, our results point to a multiscale organization of division patterns, in which vein-associated spatial information is integrated with local neighbor effects in a manner that would mitigate mechanical instability within the tissue. Together, these findings delineate a cell-size control mechanism based on coordinated divisions of distinct cell groups that supports robust morphogenesis and functional tissue design.