Quantifying metabolites using structure-switching aptamers coupled to DNA sequencing

2025/02/04 by June H. Tan, Andrew Fraser · 1 voice
Biochemistry, Genetics and Molecular Biology · #Advanced biosensing and bioanalysis techniques #DNA and Nucleic Acid Chemistry #RNA and protein synthesis mechanisms

paper · pdf · doi:10.1038/s41587-025-02554-7

openalex publication_date 2025/02/04 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

Here we report a method, smol-seq (small-molecule sequencing), using structure-switching aptamers (SSAs) and DNA sequencing to quantify metabolites. In smol-seq, each SSA detects a single target molecule and releases a unique DNA barcode on target binding. Sequencing the released barcodes can, thus, read out metabolite levels. We show that SSAs are highly specific and can be multiplexed to detect multiple targets in parallel, bringing the power of DNA sequencing to metabolomics.

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