2001/04/01 by J. Lamothe · 1 citation
Agricultural and Biological Sciences · Immunology and Microbiology · Medicine · #Insect and Pesticide Research #Research on Leishmaniasis Studies #Toxin Mechanisms and Immunotoxins
paper · doi:10.1111/j.1748-5827.2001.tb01797.x
crossref issued 2001/04/01 · crossref published 2001/04/01 · crossref published-print 2001/04/01 · openalex publication_date 2001/04/01 · crossref created 2008/04/10 · crossref deposited 2021/07/06 · openalex created_date 2025/10/10 · openalex updated_date 2026/02/24 · crossref indexed 2026/07/28
Amphotericin B was given to 19 dogs with leishmaniasis. The drug was diluted in an emulsion prepared by mixing 50 mg of amphotericin B desoxycholate with 40 ml of sterile water and 10 ml of soya bean oil solution. The dogs were infused over nearly one hour with 50 ml/kg of normal saline followed by 10 ml/kg of mannitol 20 per cent. The mixture was then loaded over 30 to 60 minutes using a syringe pump. The emulsion was given twice weekly, at an increasing dosage (from 1 to 2.5 mg/kg bodyweight), for a minimum of eight injections. All 17 dogs receiving a total dosage of more than 10 mg/kg were clinically cured by the end of the treatment, and 14 of these had a negative polymerase chain reaction test on bone marrow.