2019/04/29 by Mehmet Murat Taşkan, Mehmet Murat Taskan, Hatice Balcı Yüce +5 · 1 citation
Biochemistry, Genetics and Molecular Biology · Medicine · Neuroscience · #Antioxidants, Aging, Portulaca oleracea #Bone Metabolism and Diseases #Nitric Oxide and Endothelin Effects
paper · doi:10.1111/jre.12662
crossref issued 2019/04/29 · crossref published 2019/04/29 · crossref published-online 2019/04/29 · openalex publication_date 2019/04/29 · crossref created 2019/04/29 · crossref published-print 2019/12/01 · crossref deposited 2023/09/08 · openalex created_date 2025/10/10 · crossref indexed 2026/07/29 · openalex updated_date 2026/07/30
THE OBJECTIVE: The present study aimed to evaluate the effects of oleuropein on ligature-induced alveolar bone loss. In this respect, osteoblastic activity, osteoclastic activity, inflammatory markers, and apoptosis were evaluated. BACKGROUND: Oleuropein is a flavonoid, which has potent anti-inflammatory and bone-protective effects. METHODS: Thirty-two Wistar rats were divided into four experimental groups as following: control (C, n = 8) group; periodontitis (P, n = 8) group; periodontitis and low-dose oleuropein group (12 mg/kg/day oleuropein, LDO group, n = 8); and periodontitis and high-dose oleuropein group (24 mg/kg/day oleuropein, HDO group, n = 8). Periodontitis was induced via ligatures. Study period was 14 days, and animals were sacrificed at end of this period. Mandibles were examined via a stereomicroscope and underwent histological procedures. Osteoblast, tartrate-resistant acid phosphatase (TRAP)-positive osteoclast, and inflammatory cell counts were determined in hematoxylin-eosin stained sections. Inducible nitric oxide synthase (iNOS), bone morphogenetic protein-4, the cluster of differentiation (CD)-68, cysteine-aspartic proteases-3 (Caspase 3), and B-cell lymphoma-2 (Bcl-2) expressions were evaluated via immunohistochemistry. RESULTS: Periodontitis group had highest alveolar bone loss, and these levels significantly decreased in LDO and HDO groups. Both 12 and 24 mg/kg oleuropein groups significantly increased osteoblast cell counts and decreased TRAP-positive osteoclast and inflammatory cell counts. BMP-4 and bcl-2 expressions were elevated in oleuropein groups while caspase-3 expressions decreased. iNOS and CD68 were higher in periodontitis group compared to control group, but there was no significant difference between other groups. CONCLUSION: Oleuropein successfully decreased alveolar bone loss as a result of decreased osteoclastic activity, inflammation, and apoptosis and increased osteoblastic activity.