2001/08/12 by M. A. Kiselev, P. Lesieur, A. M. Kisselev +2
Physics and Astronomy · #physics.bio-ph #cond-mat.soft #physics.chem-ph #physics.med-ph
published as Journal of Alloys and Compounds 286 (1999) 195-202 · PDF: 14 pages, 9 figures; sourse in MS Word
arxiv created 2001/08/12 · arxiv updated 2009/11/30
The influence of dimethyl sulfoxide (DMSO) on membrane thickness, multilamellar repeat distance, and phase transitions of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) was investigated by X-ray diffraction and small-angle neutron scattering (SANS). The differential scanning calorimetry (DSC) study of water freezing and ice melting was performed in the ternary DPPC /DMSO /water and binary DMSO /water systems. The methods applied demonstrated the differences in membrane structure in three sub-regions of the DMSO mole fraction (Xdmso): from 0.0 to 0.3 for the first, from 0.3 to 0.8 for the second, and from 0.9 to 1.0 for the third sub-region. The thickness of the intermembrane solvent at T =20C decreases from 14.4 +/- 1.8 A at Xdmso =0.0 to 7.8 +/- 1.8 A at Xdmso =0.1. The data were used to determine the number of free water molecules in the intermembrane space in the presence of DMSO. The results for 0.0 < Xdmso < 0.3 were explained in the framework of DMSO-induced dehydration of the intermembrane space.