Sensitive protein alignments at tree-of-life scale using DIAMOND
2021/04/01 by Benjamin Buchfink, Klaus Reuter, Hajk‐Georg Drost · 250 citations
Biochemistry, Genetics and Molecular Biology · #Genomics and Phylogenetic Studies #Machine Learning in Bioinformatics #RNA and protein synthesis mechanisms
paper · pdf · doi:10.1038/s41592-021-01101-x
openalex publication_date 2021/04/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/30
Abstract
We are at the beginning of a genomic revolution in which all known species are planned to be sequenced. Accessing such data for comparative analyses is crucial in this new age of data-driven biology. Here, we introduce an improved version of DIAMOND that greatly exceeds previous search performances and harnesses supercomputing to perform tree-of-life scale protein alignments in hours, while matching the sensitivity of the gold standard BLASTP.
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