2025/02/01 by Tatjana Koob, Silas Aurelian Döpp, Harald Schwalbe · 1 voice
Biochemistry, Genetics and Molecular Biology · #Bacterial Genetics and Biotechnology #RNA and protein synthesis mechanisms #RNA modifications and cancer
paper · pdf · doi:10.1007/s12104-025-10217-6
openalex created_date 2025/02/01 · openalex publication_date 2025/02/01 · openalex updated_date 2026/07/27
Abstract A comprehensive understanding of RNA-based gene regulation is a fundamental aspect for the development of innovative therapeutic options in medicine and for a more targeted response to environmental problems. Within the different mechanisms of RNA-based gene regulation, riboswitches are particularly interesting as they change their structure in response to the interaction with a low molecular weight ligand, often a well-known metabolite. Four distinct classes of riboswitches recognize the very small guanidinium cation. We are focused on the Guanidine-II riboswitch with the mini-ykkC motif. We report here the assignment of the 1 H, 13 C, 15 N and 31 P chemical shifts of the 23 nucleotide-long sequence of the first stem-loop of the Guanidine-II riboswitch aptamer from Escherichia coli . Despite its small size, the assignment of the NMR signals of this RNA proved to be challenging as it has symmetrical base pairs and palindromic character.