2020/02/11 by Edel Pérez‐López, Md Musharaf Hossain, Jiangying Tu +5
paper · doi:10.1111/jeu.12784
Abstract Plasmodiophora brassicae (Wor.) is an obligate intracellular plant pathogen affecting Brassicas worldwide. Identification of effector proteins is key to understanding the interaction between P. brassicae and its susceptible host plants. To date, there is very little information available on putative effector proteins secreted by P. brassicae during a secondary infection of susceptible host plants, resulting in root gall production. A bioinformatics pipeline approach to RNA ‐Seq data from Arabidopsis thaliana (L.) Heynh. root tissues at 17, 20, and 24 d postinoculation (dpi) identified 32 small secreted P. brassicae proteins ( SSP bPs) that were highly expressed over this secondary infection time frame. Functional signal peptides were confirmed for 31 of the SSP bPs, supporting the accuracy of the pipeline designed to identify secreted proteins. Expression profiles at 0, 2, 5, 7, 14, 21, and 28 dpi verified the involvement of some of the SSP bPs in secondary infection. For seven of the SSP bPs, a functional domain was identified using Blast2 GO and 3D structure analysis and domain functionality was confirmed for SSP bP22, a kinase localized to the cytoplasm and nucleus.