3D reconstruction of histological sections: Application to mammary gland tissue

2010/03/15 by Ignacio Arganda‐Carreras, Rodrigo Fernández‐González, Arrate Muñoz‐Barrutia +2 · 8 citations
Biochemistry, Genetics and Molecular Biology · Computer Science · #AI in cancer detection #Cell Image Analysis Techniques #Medical Image Segmentation Techniques

paper · doi:10.1002/jemt.20829

openalex publication_date 2010/03/15 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/31

Abstract

In this article, we present a novel method for the automatic 3D reconstruction of thick tissue blocks from 2D histological sections. The algorithm completes a high-content (multiscale, multifeature) imaging system for simultaneous morphological and molecular analysis of thick tissue samples. This computer-based system integrates image acquisition, annotation, registration, and three-dimensional reconstruction. We present an experimental validation of this tool using both synthetic and real data. In particular, we present the 3D reconstruction of an entire mouse mammary gland and demonstrate the integration of high-resolution molecular data.

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