2011/04/28 by Shun Kageyama, Hiroko Omori, Tatsuya Saitoh +7 · 1 citation
Biochemistry, Genetics and Molecular Biology · Medicine · #Autophagy in Disease and Therapy #CRISPR and Genetic Engineering #Vibrio bacteria research studies
paper · doi:10.1091/mbc.e10-11-0893
openalex publication_date 2011/04/28 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/31
Salmonella develops into resident bacteria in epithelial cells, and the autophagic machinery (Atg) is thought to play an important role in this process. In this paper, we show that an autophagosome-like double-membrane structure surrounds the Salmonella still residing within the Salmonella-containing vacuole (SCV). This double membrane is defective in Atg9L1- and FAK family-interacting protein of 200 kDa (FIP200)-deficient cells. Atg9L1 and FIP200 are important for autophagy-specific recruitment of the phosphatidylinositol 3-kinase (PI3K) complex. However, in the absence of Atg9L1, FIP200, and the PI3K complex, LC3 and its E3-like enzyme, the Atg16L complex, are still recruited to Salmonella. We propose that the LC3 system is recruited through a mechanism that is independent of isolation membrane generation.